Aerobic and anaerobic ammonium oxidizers in the Cariaco Basin: distributions of major taxa and nitrogen species across the redoxcline

TitleAerobic and anaerobic ammonium oxidizers in the Cariaco Basin: distributions of major taxa and nitrogen species across the redoxcline
Publication TypeJournal Article
Year of Publication2017
AuthorsCernadas-Martín S, Suter EA, Scranton MI, Astor Y, Taylor GT
JournalAquatic Microbial Ecology
Volume79
Start Page31-48
Abstract

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find pdf at https://www.researchgate.net/profile/Mary_Scranton/contributions

Depth distributions of cells and functional gene copies from anaerobic ammonium-oxidizing (anammox) bacteria, aerobic ammonium-oxidizing bacteria (AOB) and archaea (AOA) in the Cariaco Basin, Venezuela were obtained using FISH and q-PCR assays. These distributions were compared to concentrations of dissolved ammonium (NH4+), nitrite (NO2−), nitrate (NO3−), hydrogen sulfide (H2S) and oxygen (O2) along the redoxcline during 3 cruises. Cell counts of anammox bacteria and copies of their nitrite reductase gene (Scalindua-nirS) were consistently observed in 2 distinct layers: the suboxic zone (≤1.1 × 106 cells l−1) and the upper euxinic zone (≤4.7 × 106 cells l−1). We hypothesize that anammox bacteria vertically organize in response to NO2− sources, with NO2− for the shallower assemblage supplied by nitrification and for the deeper assemblage by denitrification. Peaks in AOA cell abundances (up to 14.8 × 106 cells l−1) consistently coincided with copy numbers of archaeal ammonia monooxygenase subunit A gene (archaeal amoA) in the lower oxic zone. Peak abundances of beta- and gammaproteobacterial AOB cells (up to 24.0 × 106 cells l−1) and one of their ammonia monooxygenase genes (β-amoA) overlapped above the shallow anammox peak. Our results suggest that anammox bacteria AOB, AOA and denitrifiers are metabolically interdependent and ultimately controlled by vertical fluxes of O2, NO3−, NO2−, and NH4+ in the Cariaco Basin.

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